FastQCFastQC Report
Thu 2 Jun 2016
DRR024823_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024823_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318055
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT428970.41574696006175577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333860.3235687346113197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211260.20474789095425447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148110.1435444955468836No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207000.058.6707341
GTATCAA413000.044.7072871
ATCAACG505850.036.056253
TCAACGC507300.035.9344864
TATCAAC530250.034.621572
CAACGCA537500.034.00925
AACGCAG562650.032.7038466
CGCAGAG627850.028.8189838
ACGCAGA632500.028.816847
GTACATG490250.028.5418931
TACATGG483250.027.9784342
ACATGGG488200.026.5659033
GTGGTAT81150.026.5365471
GCAGAGT674550.026.3360989
TGGTATC87600.023.4522082
CATGGGA241550.023.2362384
CATGGGG217200.023.0060464
TACCTGG82050.021.9445482
CAGAGTA666000.021.48049210-11
GAGTACT424700.021.44595112-13
Produced by FastQC (version 0.11.5)