FastQCFastQC Report
Thu 2 Jun 2016
DRR024823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318055
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472880.4583034302492088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378830.3671525302007016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251800.24403824170349933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126220.12232925682214332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210850.061.9692961
GTATCAA419900.044.0662841
ATCAACG532550.034.315223
TCAACGC537450.033.9218564
TATCAAC553600.033.103842
CAACGCA558200.032.6608775
AACGCAG582200.031.3301896
GTACATG485050.029.6530171
TACATGG482350.029.0460192
ACATGGG490650.027.5102163
CGCAGAG660550.027.235598
ACGCAGA666700.027.1605837
GTGGTAT76000.025.9928321
GCAGAGT711900.024.8155489
TGGTATC82750.023.5214982
CATGGGG219450.023.5160644
CATGGGA243600.023.3456524
TACCTGG84250.022.7678932
GAGTACT459750.021.2930612-13
CAGAGTA696350.021.20553210-11
Produced by FastQC (version 0.11.5)