FastQCFastQC Report
Thu 2 Jun 2016
DRR024822_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024822_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7321018
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292800.3999443793199252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239740.32746811987076113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148420.20273136877958778No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117840.16096122151318298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138150.059.8979951
GTATCAA255000.041.7801781
TCAACGC317150.033.318864
ATCAACG316800.033.2967763
TATCAAC328300.032.1901132
CAACGCA332200.031.9210555
AACGCAG344050.030.9413996
TACCTGG66200.030.1806132
GTACATG271800.027.279271
ACGCAGA390400.027.0626857
CGCAGAG392300.026.751188
TACATGG269650.026.4299222
ACATGGG274350.025.1527563
GTACCTG84500.024.4263361
GCAGAGT424450.024.3262989
GTGGTAT53600.024.2977181
ACCTGGG80150.023.9288123
ACAACGC16300.023.0710223
TGGTATC55500.022.1077562
TATAACG8750.021.4906252
Produced by FastQC (version 0.11.5)