FastQCFastQC Report
Thu 2 Jun 2016
DRR024822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7321018
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336250.4592940489970111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282440.38579334185491687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188800.2578876325669463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93500.127714479051957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139500.057.559251
GTATCAA268000.043.6161581
ATCAACG342500.033.7011643
TCAACGC343950.033.4396824
TATCAAC351150.033.0584452
CAACGCA355500.032.3929065
TACCTGG66700.031.7205452
AACGCAG367800.031.334116
ACGCAGA425950.026.7781897
GTACATG271800.026.478771
CGCAGAG429800.026.418048
ACAACGC13000.026.4017983
TACATGG273600.025.3128262
GTACCTG86250.025.250951
GTGGTAT55800.024.2780691
GCAGAGT463300.024.1933579
ACATGGG274050.024.0531643
ACCTGGG84550.023.967223
AGAGTAC435800.021.00198410-11
TGGTATC59900.020.9574492
Produced by FastQC (version 0.11.5)