FastQCFastQC Report
Thu 2 Jun 2016
DRR024821_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024821_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11751679
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348160.29626404873720597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270010.22976291302715127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158180.13460204282298724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182250.054.821331
GTATCAA343750.042.7704051
ATCAACG422800.034.3000873
TCAACGC422850.034.22864
CAACGCA441100.032.8745545
TATCAAC446850.032.6991732
AACGCAG460350.031.5485046
ACGCAGA517300.027.7571817
CGCAGAG517400.027.504448
GTACATG337250.025.7915821
TACATGG335750.024.6441692
GCAGAGT565900.024.5990729
ACATGGG345250.023.0650673
AGAGTAC516700.020.82909410-11
GAGTACT382950.020.72203312-13
CAGAGTA554150.020.65542610-11
GTGGTAT84000.020.6540531
CATGGGG210450.019.700874
AGTACTT401350.019.25679212-13
TGGTATC87500.018.5365352
Produced by FastQC (version 0.11.5)