FastQCFastQC Report
Thu 2 Jun 2016
DRR024821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11751679
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377970.32163063677964654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296990.25272133454291934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179660.1528802820431021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187050.054.293241
GTATCAA356700.042.5546151
ATCAACG436650.034.359463
TCAACGC436450.034.3422554
CAACGCA450600.033.368135
TATCAAC458350.032.8559882
AACGCAG470150.032.1201486
ACGCAGA540300.027.7042437
CGCAGAG540050.027.5167988
GTACATG337100.025.2284451
GCAGAGT593300.024.6510379
TACATGG339750.023.7333742
ACATGGG343000.022.8093133
GAGTACT408750.020.98336412-13
AGAGTAC544200.020.87747410-11
GTGGTAT85450.020.5878181
CAGAGTA580200.020.51359210-11
CATGGGG211000.019.5355634
AGTACTT425300.019.17781812-13
GTACTTT450750.018.81964514-15
Produced by FastQC (version 0.11.5)