FastQCFastQC Report
Thu 2 Jun 2016
DRR024820_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024820_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18947607
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544760.28750860200974193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420580.2219699828057443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251200.13257610842361253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA290300.057.2022671
GTATCAA577600.044.1257171
ATCAACG692850.036.1887133
TCAACGC695100.036.0441444
TATCAAC738750.034.0885662
CAACGCA736450.034.0308655
AACGCAG765050.032.7749526
ACGCAGA839550.029.4854987
CGCAGAG837100.029.3125978
GCAGAGT918600.026.1389089
GTACATG524550.025.759621
TACATGG514650.024.9182172
ACATGGG520850.023.537173
GAGTACT626200.021.85902612-13
CAGAGTA904750.021.37564510-11
AGAGTAC852250.021.35513910-11
GTGGTAT137000.020.1788461
AGTACTT664750.019.70056312-13
GTACTTT710650.019.0664214-15
TGGTATC133600.018.9656122
Produced by FastQC (version 0.11.5)