FastQCFastQC Report
Thu 2 Jun 2016
DRR024820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18947607
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT629860.33242192536503423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT499440.26359001429573664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307300.16218406894337634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA311300.059.0221861
GTATCAA612700.043.208421
ATCAACG748050.034.9843673
TCAACGC751550.034.7771264
CAACGCA782950.033.3523835
TATCAAC799050.032.8868032
AACGCAG812850.032.1251226
ACGCAGA914850.028.2285757
CGCAGAG914150.028.0085628
GCAGAGT1002450.025.0257459
GTACATG536750.024.170431
TACATGG531100.023.1503122
ACATGGG536700.021.94513
GAGTACT686050.021.82877312-13
GTGGTAT137650.021.4949591
AGAGTAC928150.021.26680810-11
CAGAGTA988400.020.9475710-11
TGGTATC132450.020.6601032
AGTACTT724300.019.83248312-13
GTACTTT763150.019.47256514-15
Produced by FastQC (version 0.11.5)