FastQCFastQC Report
Thu 2 Jun 2016
DRR024819_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024819_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8773601
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295980.3373529295439809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229950.262093067601319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128280.14621134469187738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164750.058.1010671
GTATCAA331000.044.8697281
ATCAACG403700.036.421943
TCAACGC405550.036.255384
CAACGCA424600.034.705065
TATCAAC429650.034.300862
AACGCAG443750.033.4377336
ACGCAGA500000.029.3652827
CGCAGAG496000.029.2410248
TACCTGG67650.027.3785672
GCAGAGT529600.026.6137949
GTACATG353700.025.6805211
TACATGG338950.025.3942262
GTGGTAT65800.025.2220631
TATAACG6700.023.8553832
ACATGGG352250.023.3930023
TGGTATC66800.023.3638232
GTACCTG88650.021.690841
CAGAGTA524550.021.54349510-11
GAGTACT331200.021.391712-13
Produced by FastQC (version 0.11.5)