FastQCFastQC Report
Thu 2 Jun 2016
DRR024819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8773601
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331140.3774276947401643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261430.297973431889597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146350.16680722088911953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177300.057.702521
GTATCAA351600.044.04081
ATCAACG432350.035.4608423
TCAACGC436200.035.040274
CAACGCA453500.033.890115
TATCAAC464450.033.1314772
AACGCAG478750.032.298496
CGCAGAG535200.028.4424958
ACGCAGA540400.028.4123157
TACCTGG75050.027.2503642
GTACCGG8850.027.1063581
ACAACGC17100.026.9441033
GCAGAGT575850.026.067449
GTGGTAT73100.025.8030621
GTACATG358950.024.5310481
GTACCTG88900.023.968491
TACATGG351450.023.6779162
TGGTATC75650.023.5539652
ACATGGG354900.022.6529033
ACCTGGG86350.022.15983
Produced by FastQC (version 0.11.5)