FastQCFastQC Report
Thu 2 Jun 2016
DRR024818_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024818_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9386220
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212380.22626786928071152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163820.17453245289371014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127700.052.943131
GTATCAA241650.042.5114821
ATCAACG287800.035.1162643
TCAACGC291000.034.616664
CAACGCA306050.032.942655
TATCAAC313750.032.4239962
AACGCAG319450.031.5440716
ACGCAGA360100.027.6435647
CGCAGAG358550.027.4736618
GTACATG247300.026.254951
TACATGG249300.024.6459922
GCAGAGT396350.024.426629
GTGGTAT59400.023.3494781
ACATGGG258150.022.9252573
AGAGTAC365450.020.50555810-11
GAGTACT263500.020.4488312-13
CAGAGTA387900.020.16083710-11
CATGGGG159550.019.5037084
TGGTATC67700.018.8874452
AGTACTT278450.018.83614312-13
Produced by FastQC (version 0.11.5)