FastQCFastQC Report
Thu 2 Jun 2016
DRR024818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9386220
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236120.2515602660069762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190190.20262682954373537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106560.11352812953457302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141800.054.4016721
GTATCAA268950.042.2891961
ATCAACG319000.035.018993
TCAACGC323350.034.5648084
CAACGCA337300.033.135285
TATCAAC346550.032.560662
AACGCAG355500.031.530616
ACGCAGA402200.027.576537
CGCAGAG400300.027.4373918
GTACATG252950.025.0668541
GCAGAGT440000.024.652049
TACATGG251550.023.6996972
ACATGGG258050.022.0459793
GTGGTAT66250.021.513021
GAGTACT289500.021.2500912-13
CAGAGTA432000.020.46325710-11
AGAGTAC405900.020.19861610-11
GTACTTT314500.019.32927314-15
TGGTATC71750.019.1996632
AGTACTT303650.018.77401712-13
Produced by FastQC (version 0.11.5)