FastQCFastQC Report
Thu 2 Jun 2016
DRR024817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170641
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265130.28910738082539705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200180.2182835420119488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121830.13284785654568748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148800.055.3607141
GTATCAA295650.045.2772561
ATCAACG354800.037.2437133
TCAACGC358700.036.8381774
TATCAAC374700.035.4072072
CAACGCA377400.035.0235985
AACGCAG396750.033.466376
ACGCAGA442400.029.5660517
CGCAGAG439250.029.4444528
GCAGAGT474650.026.5454549
GTACATG308500.026.5042151
TACATGG310900.025.2833122
ACATGGG315850.023.9321083
GTGGTAT65900.023.1882761
GAGTACT310750.021.65701912-13
AGAGTAC443450.021.39727610-11
CAGAGTA470800.021.35212710-11
TGGTATC68800.021.2515372
CATGGGG162450.020.7186744
GCCGTAT13050.020.16714194
Produced by FastQC (version 0.11.5)