FastQCFastQC Report
Thu 2 Jun 2016
DRR024817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9170641
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315560.34409808431057326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250680.2733505760393412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148910.1623768720201783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161250.057.792211
GTATCAA311150.044.1466331
ATCAACG387350.035.0317343
TCAACGC389750.034.802544
TATCAAC404250.033.6835172
CAACGCA405450.033.5476385
AACGCAG425550.032.050746
CGCAGAG479400.027.890068
ACGCAGA484900.027.864787
GCAGAGT520550.025.288089
GTACATG305750.025.1864361
GTGGTAT68600.024.2066731
TACATGG308300.023.806652
ACATGGG312300.022.5231173
GAGTACT341900.021.37452512-13
AGAGTAC483800.021.26886410-11
TGGTATC73600.021.0177862
CAGAGTA514950.020.88579810-11
AGTACTT364650.019.51903212-13
GTACTTT379800.019.09927614-15
Produced by FastQC (version 0.11.5)