FastQCFastQC Report
Thu 2 Jun 2016
DRR024816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6304364
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289360.45898365005573916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213290.338321200996643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132480.2101401505369931No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94300.14957892659751246No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140550.057.3347131
GTATCAA274250.045.2065241
ATCAACG350750.035.1792533
TCAACGC351750.035.0519524
TATCAAC360850.034.1452032
CAACGCA375350.032.834775
AACGCAG385400.032.1101576
ACGCAGA424850.028.907047
CGCAGAG432150.028.2433768
GTACATG340350.027.1854761
TACATGG339250.026.6331752
GCAGAGT455750.026.0899359
ACATGGG344650.025.3681983
GTGGTAT53600.023.6829951
CATGGGA176750.022.7115364
TACCTGG51350.022.4292642
TATAACG6250.021.8125972
CAGAGTA444600.021.52224210-11
AGAGTAC427100.021.41373610-11
GAGTACT260000.021.35696612-13
Produced by FastQC (version 0.11.5)