FastQCFastQC Report
Thu 2 Jun 2016
DRR024816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6304364
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337010.5345662147680559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264600.41970926805622266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154020.24430695943317995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82460.13079828512439956No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154500.057.661491
GTATCAA300250.044.960721
ATCAACG381500.035.2714543
TCAACGC384850.034.9661034
TATCAAC394950.034.1059952
CAACGCA400900.033.519345
AACGCAG412850.032.67286
ACGCAGA463600.028.7498367
CGCAGAG471200.028.1464948
GCAGAGT500750.026.0068729
GTACATG353500.025.7602651
TACATGG351150.025.3324242
GTGGTAT59800.024.4620911
ACATGGG360650.023.7134653
GAGTACT292450.022.61075412-13
CAGAGTA489100.021.53360610-11
TACCTGG51100.021.437952
TGGTATC65650.021.4133552
GTACTTT305900.021.39381814-15
AGAGTAC471850.021.22521410-11
Produced by FastQC (version 0.11.5)