FastQCFastQC Report
Thu 2 Jun 2016
DRR024815_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024815_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460885
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350870.3708638251072706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274600.29024768824481006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164600.17397949557573103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183950.054.9010281
GTATCAA345100.041.6890951
ATCAACG419000.033.813513
TCAACGC420300.033.6975674
TATCAAC443550.032.156142
CAACGCA443400.031.9825525
AACGCAG461100.030.865036
GTACATG360100.028.8937761
TACATGG353300.028.1687832
ACGCAGA508700.027.5976817
CGCAGAG509600.027.3538138
ACATGGG361800.026.725563
GCAGAGT556850.024.5432629
CATGGGG194450.021.9259344
GAGTACT362600.021.49630512-13
GAGTACA262600.021.270011
GTGGTAT78250.021.0295371
CAGAGTA543050.020.99121710-11
AGAGTAC505550.020.71689610-11
CATGGGA135550.020.4602644
Produced by FastQC (version 0.11.5)