FastQCFastQC Report
Thu 2 Jun 2016
DRR024815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460885
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345010.36466990138871785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283200.29933774694439264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172790.18263619101172882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180100.054.0126341
GTATCAA343100.042.3090931
ATCAACG417150.034.5795633
TCAACGC419150.034.3817674
CAACGCA435650.033.090375
TATCAAC439350.032.8857962
AACGCAG455700.031.7983066
GTACATG356500.027.9463391
ACGCAGA518950.027.7034347
CGCAGAG520900.027.2564418
TACATGG350250.027.075982
ACATGGG359450.025.598163
GCAGAGT563700.024.5949959
CATGGGG195900.021.5940254
GAGTACT359100.021.33920112-13
AGAGTAC509900.020.68286110-11
CAGAGTA551600.020.38877910-11
CATGGGT76200.019.9855254
AGTACTT372000.019.68327112-13
GTGGTAT77350.019.6426771
Produced by FastQC (version 0.11.5)