FastQCFastQC Report
Thu 2 Jun 2016
DRR024814_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024814_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10660848
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339460.3184174467171842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255340.23951190374349207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152700.14323438435666658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185700.054.515261
GTATCAA355100.043.723241
ATCAACG429000.035.6352123
TCAACGC430450.035.4822434
CAACGCA451100.033.928695
TATCAAC455100.033.7596552
AACGCAG471850.032.443116
ACGCAGA521350.029.0824817
CGCAGAG518300.028.8620688
GTACATG346300.027.6853941
TACATGG335850.027.1288382
GCAGAGT564400.025.921749
ACATGGG339250.025.8154143
CATGGGG190050.022.2864554
AGAGTAC513550.021.60654310-11
GAGTACT370850.021.50579812-13
CAGAGTA554350.021.169310-11
ACAACGC23750.019.5955623
AGTACTT390600.019.41973712-13
GTGGTAT84450.019.153351
Produced by FastQC (version 0.11.5)