FastQCFastQC Report
Thu 2 Jun 2016
DRR024814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10660848
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382120.35843302521525494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296020.2776702191045215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187600.17597099217623213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192100.055.8064771
GTATCAA367300.042.813731
ATCAACG450750.034.4521453
TCAACGC451350.034.3750274
CAACGCA469800.033.0750735
TATCAAC476850.032.8227842
AACGCAG491700.031.7256056
ACGCAGA554400.027.8896437
CGCAGAG551600.027.733018
GTACATG328350.026.603731
TACATGG323000.025.7203852
GCAGAGT597400.024.9774889
ACATGGG328750.024.0835083
AGAGTAC549900.021.2977910-11
GAGTACT408250.021.27960212-13
CAGAGTA589800.020.84904910-11
TACCTGG78150.020.274232
CATGGGG184950.020.1779544
GTGGTAT85400.019.168541
AGTACTT432050.019.07400912-13
Produced by FastQC (version 0.11.5)