FastQCFastQC Report
Thu 2 Jun 2016
DRR024813_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024813_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12851920
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381050.29649266413111813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277490.21591326432159552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171620.1335364677028802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206550.055.734511
GTATCAA404150.043.3898121
ATCAACG486500.035.4351433
TCAACGC488200.035.359354
CAACGCA510300.033.9196055
TATCAAC513700.033.655112
AACGCAG535750.032.4035646
ACGCAGA593450.028.9909537
CGCAGAG594650.028.761078
GTACATG390050.027.8141161
TACATGG389850.026.7745632
GCAGAGT647100.025.812429
ACATGGG398900.024.878853
TACAACG23050.023.6621382
AGAGTAC587350.021.72862210-11
GAGTACT429750.021.1720912-13
CAGAGTA637550.020.99816510-11
CATGGGG224200.020.758644
TACCTGG98500.020.3825532
TATAACG11800.020.321472
Produced by FastQC (version 0.11.5)