FastQCFastQC Report
Thu 2 Jun 2016
DRR024813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12851920
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435950.3392100168690748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328670.25573610791228085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214310.16675329444938966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211800.054.632191
GTATCAA424100.043.5055471
ATCAACG522950.034.751063
TCAACGC523650.034.70714
CAACGCA544950.033.3931585
TATCAAC554750.032.9794462
AACGCAG568600.032.045456
ACGCAGA634450.028.421427
CGCAGAG636700.028.1411158
GTACATG389200.026.7562121
TACATGG384300.026.0063632
GCAGAGT702700.025.0296639
ACATGGG385750.024.7747273
AGAGTAC636700.022.14600810-11
TACAACG23900.021.8431952
ACAACGC24400.021.395593
GAGTACT476250.020.67919312-13
AGTACTT494800.020.3978512-13
CAGAGTA689600.020.11995510-11
CATGGGG218300.019.9249824
Produced by FastQC (version 0.11.5)