FastQCFastQC Report
Thu 2 Jun 2016
DRR024812_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024812_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12182364
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT499540.4100517764860745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332840.27321462402535335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221420.18175454287854148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227100.057.6024631
GTATCAA478500.046.648571
ATCAACG598100.036.9490553
TCAACGC601350.036.6943474
CAACGCA632200.034.9926835
TACCTGG102850.034.7868772
TATCAAC640850.034.700832
AACGCAG660950.033.4697466
ACGCAGA732600.029.7009097
CGCAGAG733500.029.2171338
GTGGTAT86350.027.3909241
GTACATG505000.027.3751831
TACATGG489650.026.8560282
GCAGAGT771950.026.842449
ACCTGGG129750.026.738433
TATAACG9750.026.5211982
ACAACGC29950.026.0554263
ACATGGG506100.024.8284453
TGGTATC94650.024.2897642
GTACCTG159050.023.5627351
Produced by FastQC (version 0.11.5)