FastQCFastQC Report
Thu 2 Jun 2016
DRR024812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12182364
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543510.4461449354164758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377870.3101778932233514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267590.21965359104357743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228400.059.2010041
GTATCAA484050.046.061161
ATCAACG613450.036.04993
TCAACGC617350.035.8403054
TACCTGG100900.034.7660982
CAACGCA641200.034.5582355
TATCAAC652700.034.0117232
AACGCAG671550.033.101436
ACAACGC24050.031.0878243
ACGCAGA748450.029.2848387
CGCAGAG748400.028.9448328
GTACATG511600.027.7081151
TACATGG491250.027.3091052
TATAACG9650.027.2878282
GCAGAGT793200.026.6225349
ACCTGGG130600.026.1397673
ACATGGG512900.025.1753833
GTGGTAT87400.024.869611
TGGTATC93800.022.408512
GTACCTG158300.022.3796061
Produced by FastQC (version 0.11.5)