FastQCFastQC Report
Thu 2 Jun 2016
DRR024811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12156926
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309340.25445577278334996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241300.19848767690121666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149470.12295048929309926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175850.056.130541
GTATCAA334750.043.7448731
ATCAACG406500.035.4346923
TCAACGC407350.035.4297034
CAACGCA430250.033.718095
TATCAAC437250.033.1399272
AACGCAG451900.032.2996866
ACAACGC25100.030.341833
ACGCAGA503150.028.7289337
CGCAGAG501300.028.4633888
TACCTGG102350.028.388952
GTACATG320250.026.9301851
TACATGG319550.025.7482622
GCAGAGT551550.024.99249
ACATGGG325250.024.1379073
TACAACG31450.024.0687712
GTACCGG12700.023.6976571
GTACCTG138050.021.596431
TATAACG10050.021.519912
AGAGTAC504850.021.4206810-11
Produced by FastQC (version 0.11.5)