FastQCFastQC Report
Thu 2 Jun 2016
DRR024811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12156926
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354360.2914881607406346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280050.230362511049257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186070.1530567842561516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180150.056.970751
GTATCAA336650.042.3260541
ATCAACG418850.033.471883
TCAACGC420250.033.309034
CAACGCA438400.032.0155565
TATCAAC448450.031.5562062
TACCTGG103500.030.9444962
AACGCAG461550.030.4401876
TATAACG11050.028.941652
ACAACGC23100.028.5031383
ACGCAGA516300.027.010757
GTACATG321750.026.6331121
CGCAGAG519650.026.5527428
TACATGG321700.025.1451382
ACATGGG324650.023.9605463
GTACCTG141450.023.7534031
GCAGAGT572650.023.4959269
TACAACG30050.022.3804172
ACCTGGG142350.021.5740833
AGAGTAC526700.021.46495810-11
Produced by FastQC (version 0.11.5)