FastQCFastQC Report
Thu 2 Jun 2016
DRR024810_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024810_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612725
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT658160.6846757813211134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466670.4854710813010879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315090.32778426512773434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271940.2828958489918312No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146190.15207966523540412No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134020.13941936339591532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA276050.057.6616821
GTATCAA549600.045.629031
TACAACG21000.036.718462
ATCAACG702700.035.4509053
TCAACGC710200.035.0233964
TATCAAC735200.033.9393462
TACCTGG79750.033.5460552
CAACGCA744500.033.4598165
AACGCAG772400.032.4212886
TATAACG10350.029.9821972
ACGCAGA849900.029.2427187
CGCAGAG847050.029.1181568
GTACATG590200.028.2683721
TACATGG582850.028.0080452
GCAGAGT898200.026.8059029
ACATGGG596700.026.535023
ACCTGGG100550.025.4343243
ACAACGC32500.025.3138433
CATGGGA346150.024.9078454
GTACAAC37650.024.105091
Produced by FastQC (version 0.11.5)