FastQCFastQC Report
Thu 2 Jun 2016
DRR024809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10540927
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT521320.4945675081517973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402820.38214855296882333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264390.2508223422854556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156000.14799457391176318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216350.061.7276881
GTATCAA429600.045.8745651
ATCAACG542050.035.9329763
TCAACGC543050.035.866644
TATCAAC565300.034.6581542
CAACGCA565450.034.42025
AACGCAG587150.033.1940886
GTACATG465600.029.753521
ACGCAGA656000.029.5230717
TACATGG455900.029.3305952
CGCAGAG660350.029.088578
ACATGGG473550.027.2020913
TACCTGG86050.026.5549982
GCAGAGT705100.026.4692179
GTGGTAT74700.024.6135791
TATAACG10600.024.3959482
CATGGGA206800.023.1653674
TGGTATC78000.022.7854752
CATGGGG234550.022.3688834
CAGAGTA683550.022.35657910-11
Produced by FastQC (version 0.11.5)