FastQCFastQC Report
Thu 2 Jun 2016
DRR024809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10540927
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT606240.5751296826170981No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478800.45422949992918077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328890.312012406498973No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124790.11838617229774952No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117480.11145129835355087No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106560.10109167817972746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105470.10005761352867731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222450.062.5446551
GTATCAA445350.045.809871
ATCAACG580300.034.8315473
TCAACGC583950.034.5839234
TATCAAC601050.033.699482
CAACGCA604550.033.444055
AACGCAG629550.032.108036
GTACATG472250.029.670441
TACATGG460300.029.0874332
ACGCAGA706250.028.3605887
CGCAGAG709350.027.982518
ACATGGG473050.027.1005233
ACAACGC21850.026.9040373
TATAACG10250.026.6111132
GTGGTAT74400.026.118511
GCAGAGT759600.025.6547979
TACCTGG86900.025.6517772
TGGTATC78000.024.0567652
CATGGGG234800.023.5703014
CATGGGA203150.022.5438924
Produced by FastQC (version 0.11.5)