FastQCFastQC Report
Thu 2 Jun 2016
DRR024808_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024808_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11562570
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300920.2602535595460179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228250.19740421030964567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132290.11441228031484349No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180800.050.823991
GTATCAA338500.041.9695851
ATCAACG399900.034.999893
TCAACGC400050.034.9629634
CAACGCA418550.033.4387555
TATCAAC422300.033.3022882
AACGCAG444900.031.6259886
ACGCAGA499900.027.947867
CGCAGAG496700.027.8753328
TACCTGG99600.025.3509182
GTACATG289700.025.2907431
GCAGAGT541200.024.9752989
TATAACG11050.024.2544442
TACATGG297150.023.4662762
ACATGGG297650.022.0979043
GTACCTG120050.021.819041
GAGTACT367150.021.19126112-13
AGAGTAC483800.021.15776310-11
CAGAGTA526800.020.62176710-11
TACAACG25350.020.2174912
Produced by FastQC (version 0.11.5)