FastQCFastQC Report
Thu 2 Jun 2016
DRR024808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11562570
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348170.30111817701427973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276610.23922882196605078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167260.14465642153950203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188800.050.895161
GTATCAA347600.041.0055121
TCAACGC421300.033.2959374
ATCAACG421950.033.2838443
CAACGCA439500.032.023575
TATCAAC445500.031.7144262
AACGCAG463450.030.5308826
TATAACG10700.028.1320152
ACAACGC22100.027.2409553
TACCTGG95550.026.8268972
ACGCAGA532900.026.3742187
CGCAGAG533850.026.0683568
GTACATG287800.023.7409761
GCAGAGT579450.023.5057289
ATAACGC13650.022.7413333
GTACCTG120100.022.2550241
TACAACG27350.022.0118872
TACATGG296350.021.901752
ACATGGG292300.021.4811293
AGAGTAC523100.021.0117610-11
Produced by FastQC (version 0.11.5)