FastQCFastQC Report
Thu 2 Jun 2016
DRR024807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14177010
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364640.2572051511566966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291950.20593199835508333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150580.10621421583253451No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA305900.039.7702331
GTATCAA503400.035.330481
ATCAACG568400.030.9519633
TCAACGC571200.030.8078174
TATCAAC592050.029.837412
CAACGCA594400.029.683935
AACGCAG617150.028.7029466
ACGCAGA678600.025.7489177
CGCAGAG673350.025.7410418
GCAGAGT731900.023.2708649
GTACATG313550.023.1719651
GAGTACT469250.022.62457312-13
TACATGG317450.021.8175372
ACATGGG317800.020.8890783
AGAGTAC602850.020.47971310-11
CAGAGTA732100.019.2681910-11
GTACTTT548900.019.26429614-15
AGTACTT513900.018.67892512-13
GCCGTAT20150.016.79178294
TCGCCGT32200.016.78345194
Produced by FastQC (version 0.11.5)