FastQCFastQC Report
Thu 2 Jun 2016
DRR024807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14177010
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417100.29420872243159873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352840.24888181640557494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189470.13364595214364663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA334950.040.5833631
GTATCAA544950.034.0879551
ATCAACG631500.029.1487143
TCAACGC633750.029.0026994
TATCAAC654100.028.3141482
CAACGCA653350.028.1037525
AACGCAG676050.027.3060676
CGCAGAG743750.024.443218
ACGCAGA749500.024.4098437
GTACATG324050.024.2339821
TACATGG325600.022.87932
GCAGAGT804750.022.193179
GAGTACT524100.022.18153412-13
ACATGGG322500.021.9180153
AGAGTAC661750.020.72842810-11
GTACTTT602800.019.191414-15
CAGAGTA802250.018.946910-11
AGTACTT567700.018.85943412-13
CATGGGG193900.017.0962964
GAGTACA260150.015.7895911
Produced by FastQC (version 0.11.5)