FastQCFastQC Report
Thu 2 Jun 2016
DRR024806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11579051
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635810.5491037218853255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472770.4082977093718647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA312600.26997031103844343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290010.2504609401927671No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157770.13625468961143708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA277350.055.875861
GTATCAA528050.043.84381
ATCAACG654500.035.044113
TCAACGC657050.034.857574
TATCAAC671200.034.2173162
CAACGCA693150.033.007675
AACGCAG721250.031.84476
TACAACG25000.029.9013232
ACGCAGA801450.028.4397967
CGCAGAG805150.028.1059918
TACCTGG101700.026.0730252
GCAGAGT853200.026.0659379
GTGGTAT117300.025.2927761
TATAACG11300.024.9635352
GTACATG543900.024.810071
TACATGG545800.024.0155492
TGGTATC121150.023.2453822
ACAACGC35550.022.7448313
ACATGGG554050.022.586713
GAGTACT548800.021.83325412-13
Produced by FastQC (version 0.11.5)