FastQCFastQC Report
Thu 2 Jun 2016
DRR024805_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024805_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17978720
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT684710.38084468749721895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516240.28713946265362605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310370.17263186700721744No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188220.10469043402422419No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA371150.050.3091281
GTATCAA701900.042.419151
ATCAACG841150.035.0422943
TCAACGC845450.034.8137324
TATCAAC878300.033.7185022
CAACGCA886300.033.2407955
AACGCAG923500.031.9767366
ACGCAGA1025500.028.6213877
CGCAGAG1031850.028.2166868
GCAGAGT1103450.025.853389
GTACATG628050.024.7067741
TACATGG624400.023.9368572
ACATGGG640350.022.5161863
GAGTACT718200.021.51939812-13
GTGGTAT179700.021.4304941
TATAACG16900.021.4211882
AGAGTAC1004250.021.1555710-11
CAGAGTA1091650.020.77059610-11
TGGTATC184200.019.883252
AGTACTT742050.019.86505512-13
Produced by FastQC (version 0.11.5)