FastQCFastQC Report
Thu 2 Jun 2016
DRR024805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17978720
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT806820.44876387195528933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635750.35361249299171466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404390.22492702483825322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA400300.052.9169961
GTATCAA751450.041.6887631
ATCAACG928450.033.3946883
TCAACGC933500.033.168654
TATCAAC954950.032.5615462
CAACGCA968250.031.9974065
AACGCAG1008800.030.753096
ACGCAGA1134850.027.1086527
CGCAGAG1135600.026.9220588
GTACATG614200.025.2265131
GCAGAGT1214900.024.76629
TACATGG616100.023.9908712
ACATGGG630900.022.5261383
GTGGTAT185400.021.4955711
GAGTACT827250.021.4192512-13
AGAGTAC1107050.021.09362810-11
CAGAGTA1202400.020.63092810-11
AGTACTT851950.019.84109712-13
GTACTTT888050.019.83563814-15
TGGTATC192150.019.701912
Produced by FastQC (version 0.11.5)