FastQCFastQC Report
Thu 2 Jun 2016
DRR024804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15964926
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563770.35313035588138647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383430.24017023317239303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247490.1550210755752955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA255400.055.771811
GTATCAA529000.046.216861
ATCAACG650950.037.115073
TCAACGC652000.037.0548364
CAACGCA683900.035.3738945
TATCAAC688750.035.2660642
AACGCAG714300.033.9991876
ACGCAGA795350.030.3324997
CGCAGAG796900.030.095838
GTACATG485150.027.4119361
GCAGAGT868200.027.158619
TACATGG471800.026.6581522
ACATGGG485550.024.8742183
AGAGTAC802650.022.27368710-11
GTGGTAT114450.021.7769181
CAGAGTA853250.021.33285710-11
GAGTACT615400.021.18850312-13
CATGGGG271950.020.5026264
AGTACTT642050.020.2065412-13
TGGTATC116800.019.6847632
Produced by FastQC (version 0.11.5)