FastQCFastQC Report
Thu 2 Jun 2016
DRR024804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15964926
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT641080.40155525932284314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447510.28030822065821037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302620.18955302392256626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA276850.056.4970781
GTATCAA563350.046.1324231
ATCAACG704300.036.552493
TCAACGC710550.036.2053344
CAACGCA736100.034.986325
TATCAAC741950.034.875132
AACGCAG771300.033.541946
ACGCAGA872400.029.4432727
CGCAGAG870250.029.3235118
GCAGAGT947850.026.5408929
GTACATG477350.025.8269981
TACATGG466200.025.1590422
ACATGGG475350.023.3885823
AGAGTAC883150.022.02360210-11
ACAACGC26200.021.8992083
GAGTACT687550.021.34900912-13
CAGAGTA936350.021.18900310-11
GTGGTAT119200.020.5670011
AGTACTT710700.020.55439412-13
CATGGGG273200.019.8965444
Produced by FastQC (version 0.11.5)