FastQCFastQC Report
Thu 2 Jun 2016
DRR024803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18131432
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674500.37200591767930963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486480.268307544599897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312360.17227541652529155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA318100.057.5062641
GTATCAA640600.045.7330671
ATCAACG808500.035.8603133
TCAACGC815500.035.5001224
TATCAAC859150.033.9025232
CAACGCA904700.032.0256655
AACGCAG929850.031.2489936
ACGCAGA979900.029.2774897
CGCAGAG1006000.028.3250568
GTACATG796700.026.4194891
GCAGAGT1060750.026.2737269
TACATGG766500.026.272152
GTGGTAT121600.025.9875221
ACATGGG811350.024.1570453
TGGTATC133250.022.6530912
CAGAGTA1055600.021.22615210-11
CATGGGG515400.020.8975244
AGAGTAC1018850.020.8708610-11
GAGTACT678900.020.51853812-13
GTACTTT675500.020.33279814-15
Produced by FastQC (version 0.11.5)