FastQCFastQC Report
Thu 2 Jun 2016
DRR024803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18131432
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT777240.4286699473047688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT576290.3178403117856328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391960.21617707856720858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA338650.058.8311541
GTATCAA681100.045.7978131
ATCAACG876450.035.276443
TCAACGC886250.034.9074944
TATCAAC927800.033.5267942
CAACGCA937950.033.003085
AACGCAG961750.032.117746
ACGCAGA1075150.028.5267727
CGCAGAG1103700.027.6576188
GTACATG814750.025.8854121
GCAGAGT1167150.025.7995959
TACATGG782350.025.4405652
GTGGTAT124500.024.4157871
ACATGGG823600.023.3840523
TGGTATC132600.021.8483872
AGAGTAC1116750.021.11826510-11
CAGAGTA1158900.020.88154610-11
CATGGGG520000.020.762694
GAGTACT762550.020.42250612-13
AGTACTT747050.020.37439312-13
Produced by FastQC (version 0.11.5)