FastQCFastQC Report
Thu 2 Jun 2016
DRR028603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684789
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15250.049.7512554
ATCAACG16400.046.26263
GGTATCA12600.044.5821231
CAACGCA18000.041.745085
GTATCAA20250.040.349071
GTATACG2250.038.9080351
AACGCAG19650.038.2386136
TATCAAC23100.033.4760672
TCTCGGG13700.033.003773145
ACGCAGA21850.032.385297
GTACATG33650.032.086121
CGCAGAG21900.031.6432278
TCGCCGG9050.030.135519145
TACATGG37150.027.8849642
ACATGGG37700.025.736583
CTTAGGG19700.025.137754145
AGTACCG1851.7557325E-423.660295
GCAGAGT32900.020.8404459
CATGGGG29200.019.9870054
GAGTACA30350.019.9507851
Produced by FastQC (version 0.11.5)