FastQCFastQC Report
Thu 2 Jun 2016
DRR028603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684789
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.071.952321
GTATCAA36750.055.9707031
ATCAACG39750.051.2023853
TCAACGC39900.050.8283654
CAACGCA42600.047.606855
AACGCAG44450.045.951366
TATCAAC45650.044.90212
ACGCAGA49200.041.6622127
CGCAGAG49350.041.3888138
GTACATG36050.035.0812951
GCAGAGT61350.032.230659
TACATGG40650.029.043442
TGGTATC13050.027.1960962
ACATGGG41650.026.7809943
GTGGTAT15000.026.650361
CAGAGTA56650.021.2240949
TCTATAC10350.020.2945143
CATGGGG31050.019.5947044
GTATAGG7600.019.1270521
GAGTACA33350.018.960731
Produced by FastQC (version 0.11.5)