FastQCFastQC Report
Thu 2 Jun 2016
DRR028602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580519
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12650.048.4768751
TCAACGC15550.047.41734
ATCAACG16250.045.374713
CAACGCA19250.038.303335
GTATCAA19550.037.7155531
GTACATG35050.036.033291
AACGCAG21600.033.7980886
ACGCAGA22050.033.1061947
CGCAGAG21800.032.4791878
TATCAAC23650.031.1771282
CGTAATG1203.897652E-430.418282
TACATGG42100.029.9992142
CTTAGGG17900.029.639763145
TCTCGGG15000.027.244553145
ACATGGG44950.025.9858043
GCGTTAC2258.583538E-725.9552577
GAGTACA32200.023.8056091
GTGTACG2502.1473315E-623.3612381
TCTAGCG2202.1135602E-523.2285023
CATGGGA17100.023.0538524
Produced by FastQC (version 0.11.5)