FastQCFastQC Report
Thu 2 Jun 2016
DRR028602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580519
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.11312739676017813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.10692690185945249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.082.080331
GTATCAA39900.061.433971
TCAACGC42950.055.3064654
ATCAACG43650.054.5854453
CAACGCA47000.050.3866045
AACGCAG49250.048.3787776
TATCAAC50150.048.232612
CGCAGAG53800.043.7488148
ACGCAGA55050.043.1500937
GCAGAGT64850.035.4009139
GTACATG36100.031.8347531
TACATGG41100.026.6072712
ACATGGG42150.025.085373
GTGGTAT10750.025.034841
TGGTATC10900.023.2544882
CAGAGTA57450.022.3125119
CCGTGCA5900.022.0945789
CGCTATA2003.1270666E-421.7263362
GTATAGG6850.021.2368931
GTATTAG13350.020.1591421
Produced by FastQC (version 0.11.5)