FastQCFastQC Report
Thu 2 Jun 2016
DRR028601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198770
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.061.9599951
GTATCAA56200.050.935081
TCAACGC54900.048.682854
ATCAACG56400.047.6470453
GTACATG119300.039.8481181
TATCAAC69450.039.53512
CAACGCA69250.038.4881025
TACATGG121300.037.385062
AACGCAG74150.035.9435776
ACGCAGA74000.035.5213627
CGCAGAG75000.034.7512478
ACATGGG127000.034.039663
CATGGGA62600.029.5130064
CTTAGGG40250.028.310936145
GCAGAGT89650.027.5238489
CATGGGG75400.025.3744854
GAGTACA95400.024.8772431
AGTACAT94900.024.1618792
TCTCGGG42450.023.97362145
TACACGC7350.022.8504055
Produced by FastQC (version 0.11.5)