FastQCFastQC Report
Thu 2 Jun 2016
DRR028601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198770
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.110886371949218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.10729124007040206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44800.082.402311
GTATCAA89600.060.9095841
TCAACGC103300.051.949624
ATCAACG104150.051.8733183
CAACGCA111000.048.411155
TATCAAC114500.047.374082
AACGCAG115650.046.4020356
ACGCAGA129300.041.0553677
CGCAGAG132500.039.845218
GTACATG120950.036.7704771
GCAGAGT146150.034.6867569
TACATGG125950.034.384882
GTGGTAT23400.031.9871161
ACATGGG130800.031.7810763
TGGTATC23250.029.2798842
GTACGGA5200.027.8542296
CATGGGA66400.027.5941434
GAGTACA98350.025.0483461
AGTACAT96700.024.4149362
CAGAGTA147050.022.1621539
Produced by FastQC (version 0.11.5)