FastQCFastQC Report
Thu 2 Jun 2016
DRR028600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706058
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.046.573321
TCAACGC18500.046.1453484
ATCAACG19400.044.004583
GTATCAA23050.039.5687981
GTACATG38800.037.798811
CAACGCA22450.037.70015
AACGCAG24400.034.984126
TACATGG40250.033.3553662
TATCAAC27400.032.4880072
ACGCAGA26750.031.635187
CGCAGAG26350.031.007068
TCTCGGG15750.029.158915145
ACATGGG44550.028.9894073
CGGTGGG17600.022.832174145
GTCGTAC1600.002096416422.801521
TAATACG4709.094947E-1221.7342154
CATGGGG33300.021.6922574
TAATCCG1700.00298106421.4602554
GCAGAGT35400.020.8126969
CTTAGGG19550.019.8207145
Produced by FastQC (version 0.11.5)