FastQCFastQC Report
Thu 2 Jun 2016
DRR028600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706058
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.069.581341
GTATCAA37050.055.528281
TCAACGC40350.049.902624
ATCAACG40450.049.779253
CAACGCA43800.045.8065645
AACGCAG46000.043.7732776
TATCAAC48800.042.003812
ACGCAGA51950.038.4809347
CGCAGAG52850.037.825638
GTGGTAT11650.031.8244021
GCAGAGT61800.031.5272379
GTACATG37850.031.4987981
TACATGG39800.028.3898852
GGACCGC2051.30057615E-524.7324036
TGGTATC14750.024.0616912
GTCGTAC1550.001780684623.450621
ACATGGG44850.023.255313
CAGAGTA59150.022.5312429
GAGTACA34400.021.9781381
AGTACAT32800.020.9783762
Produced by FastQC (version 0.11.5)