FastQCFastQC Report
Thu 2 Jun 2016
DRR028599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651423
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19950.050.4593544
ATCAACG21350.047.1505433
GTATCAA23950.045.0776941
GGTATCA15250.043.528841
CAACGCA23550.043.0555765
AACGCAG24600.041.2165686
TATCAAC27250.037.744892
ACGCAGA26750.037.6311457
CGCAGAG27900.035.553868
GTACATG39450.034.393111
TACATGG41900.030.2928922
CTTAGGG16250.027.828632145
ACATGGG43350.026.5872553
GCAGAGT36600.024.7111349
TATACGT1600.00209942822.7958374
CATGGGG35250.021.3149174
CAGCGTA2856.742175E-620.473749
GAGTACA34550.020.0576971
CGGTGGG16850.020.021778145
GTGGTAT12400.019.4132291
Produced by FastQC (version 0.11.5)