FastQCFastQC Report
Thu 2 Jun 2016
DRR028599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651423
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.1145678605663116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.10433426202735459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.076.122411
GTATCAA43600.057.893661
TCAACGC48700.051.315264
ATCAACG49350.050.6393783
CAACGCA51850.048.3374525
TATCAAC53450.046.754972
AACGCAG53750.046.628786
ACGCAGA60900.040.797477
CGCAGAG61800.039.617288
GCAGAGT71900.033.3469059
GTACATG42950.032.518171
TACATGG44500.029.6256452
GTGGTAT12350.028.2723751
ACATGGG46100.026.0833633
TGGTATC13000.023.9597072
GTCTAGC3757.479684E-921.337791
CAGAGTA68050.020.8633659
GAGTACA35650.020.8127121
GTATAGA8800.020.6654741
GTATATA13050.020.62431
Produced by FastQC (version 0.11.5)